2C9A
Crystal structure of the MAM-Ig module of receptor protein tyrosine phosphatase mu
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-29 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.856, 97.737, 134.357 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.225 |
Rwork | 0.222 |
R-free | 0.27500 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.594 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.140 | 0.530 |
Number of reflections | 10567 | |
<I/σ(I)> | 12.7 | 4.1 |
Completeness [%] | 99.3 | 99.3 |
Redundancy | 10.5 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 295 | 0.1 M TRI-SODIUM CITRATE, PH 6.2 0.1 M AMMONIUM CHLORIDE 30% (W/V) PEG 4000 |