2C98
Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.097, 113.097, 39.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.900 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjw |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.747 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 22773 | |
| <I/σ(I)> | 22 | 3.8 |
| Completeness [%] | 98.9 | 95.7 |
| Redundancy | 7.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 5% MPD |
