2C8L
Crystal Structure of (SR) Calcium-ATPase E2(Tg) form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-27 |
Detector | MARRESEACH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.514, 71.514, 589.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.990 - 3.100 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.30600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iwo |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 3.170 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.100 | 0.730 |
Number of reflections | 29437 | |
<I/σ(I)> | 20.8 | 2.79 |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 50 MM NAOAC, 15% PEG2000 MME, 10& GLYCEROL, 4% MPD, pH 6.80 |