2C8H
Structure of the PN loop Q182A mutant C3bot1 Exoenzyme (NAD-bound state, crystal form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.388, 75.611, 120.783 |
| Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
| Resolution | 31.780 - 1.650 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.780 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.060 |
| Number of reflections | 107532 |
| <I/σ(I)> | 18.6 |
| Completeness [%] | 96.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
