2C8E
Structure of the ARTT motif E214N mutant C3bot1 Exoenzyme (Free state, crystal form III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.674, 70.115, 112.882 |
| Unit cell angles | 90.00, 98.63, 90.00 |
Refinement procedure
| Resolution | 37.270 - 1.600 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.270 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.050 |
| Number of reflections | 87212 |
| <I/σ(I)> | 24.3 |
| Completeness [%] | 96.0 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22.5% PEG 3350 W/W, 100 MM LI2SO4, 100 MM SODIUM CITRATE PH 3.0,3-10% MPEG 550 V/V |
