2C88
Crystal Structure Of (SR) Calcium-ATPase E2(Tg):AMPPCP form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-27 |
| Detector | MARRESEACH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.529, 71.529, 590.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 3.100 |
| R-factor | 0.261 |
| Rwork | 0.261 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.170 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.120 | 0.600 |
| Number of reflections | 29134 | |
| <I/σ(I)> | 19.6 | 3.7 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 50 MM SODIUM ACETATE, 15 % PEG 2000 MME, 10 % GLYCEROL, 4 % MPD, pH 6.80 |
