2C86
x-ray structure of the N and C-terminal domain of coronavirus nucleocapsid protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.399, 96.662, 66.593 |
| Unit cell angles | 90.00, 98.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.269 |
| Rwork | 0.266 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1btl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.346 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.500 |
| Number of reflections | 8739 | |
| <I/σ(I)> | 16.3 | 3.1 |
| Completeness [%] | 96.9 | 96.3 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 20% PEG 3350, 0.2M LITHIUM SULFATE, pH 7.40 |
