2C83
CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRUCKER |
| Wavelength(s) | 0.9713700, 0.9790300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.930, 60.984, 64.635 |
| Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.22690 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.962 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 1.400 |
| Number of reflections | 30006 | |
| <I/σ(I)> | 15.4 | 2.1 |
| Completeness [%] | 98.7 | 93.1 |
| Redundancy | 5.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
