2C81
Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-08 |
Detector | RIGAKU-MSC RAXIS IV |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 73.750, 73.750, 162.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.370 - 1.700 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c7t |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.370 |
Number of reflections | 52335 | |
<I/σ(I)> | 10 | |
Completeness [%] | 91.5 | 98.9 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1M NAHEPES, K,NA TARTRATE TETRAHYDRATE PH7.5 |