2C7Q
HhaI DNA methyltransferase complex with oligonucleotide containing 2- aminopurine outside the recognition sequence (paired with G) and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-30 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 95.100, 95.100, 312.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.980 - 1.850 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mht |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 1.960 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.070 | 0.200 |
Number of reflections | 45796 | |
<I/σ(I)> | 8.5 | 3.7 |
Completeness [%] | 98.7 | 94.5 |
Redundancy | 8.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 50 MM NA CITRATE PH 5.6, 1.6 M AMMONIUM SULFATE |