2C7K

Laue structure of phycoerythrocyanin from Mastigocladus laminosus

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	288
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 63
Unit cell lengths	156.745, 156.745, 40.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.300 - 3.200
R-factor	0.192
Rwork	0.192
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c7j
RMSD bond length	0.007
RMSD bond angle	1.241
Data reduction software	LaueView
Data scaling software	LaueView
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.200	3.400
High resolution limit [Å]	3.200	3.200
Rmerge	0.160
Number of reflections	5868
Completeness [%]	60.7	30.6
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	5% PEG, 5MM POTASSIUM PHOSPHATE, PH 8.5, 4 DEG C, 4 MICRO-LITER PROTEIN, HANGING DROP