2C7F
The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5-alpha-L-Arabinotriose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 173.341, 173.341, 272.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 145.860 - 2.700 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.25500 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.558 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.590 |
| Number of reflections | 113914 | |
| <I/σ(I)> | 11.2 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.0M SODIUM ACETATE,0.1M SODIUM CACODYLATE PH 6.5,5% DIOXANE |
