2C6X
Structure of Bacillus subtilis citrate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.504, 185.563, 82.563 |
| Unit cell angles | 90.00, 106.62, 90.00 |
Refinement procedure
| Resolution | 29.810 - 3.400 |
| R-factor | 0.284 |
| Rwork | 0.284 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a59 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.130 | 0.370 |
| Number of reflections | 20446 | |
| <I/σ(I)> | 4.72 | 2.17 |
| Completeness [%] | 96.4 | 91.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M LI2SO4, 0.1M TRIS PH8.0, 30%PEG4K, pH 8.00 |
