2C4T
X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with an inhibitor, an analogue of S- adenosyl methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 130 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-18 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.319, 129.505, 182.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.290 - 2.300 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c2w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.140 | 0.660 |
| Number of reflections | 40659 | |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 32% PEG 1K 100MM PHOSPHATE CITRATE PH4.2 220MM LI2SO4, pH 4.00 |
