2C4P
Crystal structure of human ubiquitin-conjugating enzyme UbcH5A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-07 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.621, 50.565, 66.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.140 - 2.350 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qcq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.371 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.150 | 2.470 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.060 | 0.510 |
Number of reflections | 89448 | |
<I/σ(I)> | 12.5 | 2 |
Completeness [%] | 96.8 | 87.7 |
Redundancy | 2.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 200 MM AMMONIUM SULPHATE, 100 MM SODIUM ACETATE TRIHYDRATE [PH 4.6], 25% W/V PEG MME 2000 |