2C4D
2.6A Crystal Structure of Psathyrella velutina Lectin in Complex with N-acetylglucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 106.767, 106.767, 86.622 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.450 - 2.600 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bwm |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.722 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.640 | 2.670 |
| High resolution limit [Å] | 2.500 | 2.600 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 17901 | |
| <I/σ(I)> | 5.47 | 1.57 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.13 | 11.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.2M AMMONIUM SULPHATE 0.1M MES PH 6.5 |
