2C47
Structure of casein kinase 1 gamma 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.959, 97.088, 185.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2CSN |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.466 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.480 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 66823 | |
| <I/σ(I)> | 11.93 | 2.39 |
| Completeness [%] | 99.1 | 93.3 |
| Redundancy | 4.04 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M NACACOD PH=6.5 20% PEG10K 0.2 M MGCL2, pH 6.50 |
