2C40
CRYSTAL STRUCTURE OF INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE FROM BACILLUS ANTHRACIS AT 2.2A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.154, 78.154, 203.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.880 - 2.200 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hoz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.781 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.810 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 37372 | |
| <I/σ(I)> | 23.7 | 3.02 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 22% PEG 3350, 0.2 M NACL, TRIS-HCL PH 8.5 |
