2C3B
The Crystal Structure of Aspergillus fumigatus Cyclophilin reveals 3D Domain Swapping of a Central Element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.829, 64.829, 156.288 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.900 - 1.850 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.21400 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.947 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 33079 | |
| <I/σ(I)> | 19.2 | 2.6 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | DROP SOLUTION: 13% AMMONIUM SULFATE, 0.1 M MES PH 6. RESERVOIR SOLUTION: 50% AMMONIUM SULFATE, 0.1 M SODIUM CITRATE PH 5.5. |
