2C39
RNase PH core of the archaeal exosome in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 206.200, 214.000, 432.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.250 - 3.300 |
| R-factor | 0.275 |
| Rwork | 0.274 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.224 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.180 | 0.750 |
| Number of reflections | 129777 | |
| <I/σ(I)> | 8.1 | 2.4 |
| Completeness [%] | 93.8 | 95.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 50 MM MES, 2.4 M SODIUM MALONATE, PH 7.0 |
