2C39
RNase PH core of the archaeal exosome in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-17 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 206.200, 214.000, 432.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 93.250 - 3.300 |
R-factor | 0.275 |
Rwork | 0.274 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2br3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.224 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.180 | 0.750 |
Number of reflections | 129777 | |
<I/σ(I)> | 8.1 | 2.4 |
Completeness [%] | 93.8 | 95.3 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 50 MM MES, 2.4 M SODIUM MALONATE, PH 7.0 |