2C2P
The crystal structure of mismatch specific uracil-DNA glycosylase (MUG) from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-06 |
Detector | ADSC CCD |
Spacegroup name | P 6 |
Unit cell lengths | 101.733, 101.733, 37.561 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 87.710 - 1.750 |
R-factor | 0.183 |
Rwork | 0.179 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mug |
RMSD bond length | 0.021 |
RMSD bond angle | 1.916 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.050 | 0.530 |
Number of reflections | 22686 | |
<I/σ(I)> | 18.8 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M SODIUM ACETATE TRIHYDRATE, 0.1 M SODIUM CACODYLATE PH 6.5, 30% W/V PEG 8000 |