2C2F
Dps from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 90.616, 90.616, 90.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.020 - 1.610 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.15300 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.637 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.910 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.570 |
| Number of reflections | 32579 | |
| <I/σ(I)> | 56.5 | 11.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 84 | 84.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 200MM LITHIUM SULFATE, 100MM TRIS-HCL PH8.5, 15% PEG4000, pH 8.00 |
