2C29
Structure of dihydroflavonol reductase from Vitis vinifera at 1.8 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.818, 89.927, 93.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.430 - 1.810 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.428 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.910 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.070 | 0.590 |
| Number of reflections | 66981 | |
| <I/σ(I)> | 6.7 | 2 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 5.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | NACL 80-200MM PEG 3350 25-30% HEPES 100MM PH 7.5 |
