2C1Y
Structure of PDI-related Chaperone, Wind mutant-Y55K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.421, 119.095, 64.034 |
| Unit cell angles | 90.00, 100.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ovn |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.060 | 0.270 |
| Number of reflections | 22938 | |
| <I/σ(I)> | 16.83 | 3.51 |
| Completeness [%] | 97.8 | 91.3 |
| Redundancy | 4.67 | 2.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 0.1M TRIS-HCL PH 8.2-8.8 25 MM MGCL2 10% PEG4000 5% GLYCEROL |
