2C1X
Structure and activity of a flavonoid 3-O glucosyltransferase reveals the basis for plant natural product modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.014, 93.394, 106.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.190 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.515 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.190 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.470 |
| Number of reflections | 37371 | |
| <I/σ(I)> | 13 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG 10000, 0.1M BIS-TRIS PROPANE PH 7.0, 0.5% V/V PLURONIC ACID |
