2C1Q

X-ray structure of biotin binding protein from chicken

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-21
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	61.653, 61.653, 179.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.100
R-factor	0.196
Rwork	0.195
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wbi
RMSD bond length	0.014
RMSD bond angle	1.593
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.010	0.050
Number of reflections	10571
<I/σ(I)>	14	4
Completeness [%]	99.9	100
Redundancy	8.6	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1