2C1N
Molecular basis for the recognition of phosphorylated and phosphoacetylated histone H3 by 14-3-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.135, 72.375, 71.171 |
| Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
| Resolution | 69.340 - 2.000 |
| R-factor | 0.258 |
| Rwork | 0.255 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qja |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.511 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.500 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 37201 | |
| <I/σ(I)> | 5.5 | 2.3 |
| Completeness [%] | 77.9 | 77.9 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100MM HEPES PH 7.0, 23% ETHYLENE GLYCOL |
