2C1L
Structure of the BfiI restriction endonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-09 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 138.925, 138.925, 94.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.010 - 1.900 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.21700 |
Structure solution method | MIRAS |
RMSD bond length | 0.017 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.260 |
Number of reflections | 70374 | |
<I/σ(I)> | 10.4 | 2.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | MES 0.1M PH 6.5 K/NA TARTRATE 1.1M GLYCEROL 25% |