2C1J
Molecular basis for the recognition of phosphorylated and phosphoacetylated histone H3 by 14-3-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-05 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.191, 72.413, 71.160 |
Unit cell angles | 90.00, 102.96, 90.00 |
Refinement procedure
Resolution | 69.340 - 2.600 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qja |
RMSD bond length | 0.013 |
RMSD bond angle | 1.527 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.200 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 0.480 |
Number of reflections | 21834 | |
<I/σ(I)> | 6.4 | 1.5 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1 M HEPES PH7.0, 23% ETHYLENE GLYCOL, pH 7.00 |