2C1F
The structure of the family 11 xylanase from Neocallimastix patriciarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-14 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 117.336, 117.336, 117.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.050 - 2.100 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f5j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.218 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.040 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 15357 | |
| <I/σ(I)> | 7.37 | 2.07 |
| Completeness [%] | 96.8 | 82.5 |
| Redundancy | 7.49 | 2.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M CDCL2, 0.1M SODIUM ACETATE AND 30% V/V PEG400 |
