2C0H
X-ray structure of beta-mannanase from blue mussel Mytilus edulis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.073, 65.164, 93.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.600 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.170 | 0.310 |
| Number of reflections | 48523 | |
| <I/σ(I)> | 11.6 | 4.9 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 5.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH6.5, 0.2 M AMMONIUM SULPHATE, 25%(W/V) MME PEG 5000, 3% DIOXANE, pH 6.50 |
