2C0D
Structure of the mitochondrial 2-cys peroxiredoxin from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-17 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.632, 76.534, 60.154 |
| Unit cell angles | 90.00, 113.34, 90.00 |
Refinement procedure
| Resolution | 59.870 - 1.780 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e2y |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.220 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.090 | 0.250 |
| Number of reflections | 41762 | |
| <I/σ(I)> | 13.45 | 2.58 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.61 | 3.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2M AMMONIUM SULFATE, 0.1M BIS-TRIS 6.5, 25% PEG 3350, pH 6.50 |
