2C04
GMPPCP complex of SRP GTPase Ffh NG Domain at ultra-high resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-26 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.006, 54.440, 98.831 |
| Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
| Resolution | 19.610 - 1.150 |
| R-factor | 0.146 |
| Rwork | 0.143 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jpn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.487 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 192311 | |
| <I/σ(I)> | 29.7 | 3.4 |
| Completeness [%] | 94.3 | 90.9 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.7 | 30% MPD, 0.1M NAACETATE, PH4.7, 20MM CACL2, 0.15M KOACETATE, 2MM MGCL2, 2MM GMPPCP |
