2C03
GDP COMPLEX OF SRP GTPASE FFH NG DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.590, 55.110, 96.110 |
| Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.240 |
| R-factor | 0.138 |
| Rwork | 0.134 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ffh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.396 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.063 | 0.470 |
| Number of reflections | 154602 | |
| <I/σ(I)> | 12.5 | 2 |
| Completeness [%] | 94.6 | 96.1 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 30% DIOXANE, 2MM GDP |
