2BZF
Structural basis for DNA bridging by barrier-to-autointegration factor (BAF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-12 |
| Detector | RIGAKU/MSC RAXIS IV |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.830, 45.830, 241.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.870 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ci4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.110 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 6542 | |
| <I/σ(I)> | 19.2 | 5.05 |
| Completeness [%] | 97.9 | 97.2 |
| Redundancy | 4.89 | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | pH 7.40 |
