2BZF

Structural basis for DNA bridging by barrier-to-autointegration factor (BAF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2005-08-12
Detector	RIGAKU/MSC RAXIS IV
Spacegroup name	P 43 21 2
Unit cell lengths	45.830, 45.830, 241.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.870
R-factor	0.244
Rwork	0.244
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ci4
RMSD bond length	0.008
RMSD bond angle	1.110
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.870	2.870
Rmerge	0.070	0.290
Number of reflections	6542
<I/σ(I)>	19.2	5.05
Completeness [%]	97.9	97.2
Redundancy	4.89	3.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		pH 7.40