2BZD
Galactose recognition by the carbohydrate-binding module of a bacterial sialidase.
Replaces: 2BQ9Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 141.457, 141.457, 158.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 122.170 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.193 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w8o |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.603 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.100 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.150 | 0.440 |
| Number of reflections | 121364 | |
| <I/σ(I)> | 9.9 | 2.8 |
| Completeness [%] | 98.3 | 99.6 |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16 % PEG 3350, 0.2 M DI-AMMONIUM HYDROGEN CITRATE |
