2BZ8
N-terminal Sh3 domain of CIN85 bound to Cbl-b peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER |
| Synchrotron site | SRS |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Detector | BRUKER |
| Spacegroup name | P 65 |
| Unit cell lengths | 51.158, 51.158, 97.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | |
| Number of reflections | 9738 | |
| <I/σ(I)> | 29 | 2.87 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.8 M NA CITRATE, 0.1 M BIS-TRIS PH 7.5, 0.2 M NACL |
