2BZ1
CRYSTAL STRUCTURE OF APO E. COLI GTP CYCLOHYDROLASE II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-22 |
Detector | ADSC CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 71.920, 71.920, 128.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.570 - 1.540 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21200 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.600 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.040 | 0.250 |
Number of reflections | 27407 | |
<I/σ(I)> | 76.4 | 2.3 |
Completeness [%] | 89.4 | 26.5 |
Redundancy | 17.2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 1.5M AMMONIUM SULPHATE, 0.1M NA HEPES PH 7.0, 2% PEG 400, 15% GLYCEROL |