2BYS
CRYSTAL STRUCTURE OF ACHBP FROM APLYSIA CALIFORNICA IN complex with lobeline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.764, 85.731, 117.318 |
| Unit cell angles | 89.95, 97.33, 106.64 |
Refinement procedure
| Resolution | 47.730 - 2.050 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2byn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.162 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.060 | 0.260 |
| Number of reflections | 168545 | |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 95.8 | 95.4 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25% PEG 4K, 0.1 M HEPES, PH 7.5 |
