2BYN
Crystal structure of apo AChBP from Aplysia californica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 144.153, 146.768, 143.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.030 - 2.020 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ux2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.411 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.020 |
Rmerge | 0.060 |
Number of reflections | 97846 |
<I/σ(I)> | 13.6 |
Completeness [%] | 98.5 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 12-14% PEG 4000, 0.1 M SODIUM CITRATE, PH 5.6, 20% ISOPROPANOL, 5% GLYCEROL |