2BYM
Histone fold heterodimer of the Chromatin Accessibility Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 130.550, 130.550, 59.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.960 - 2.800 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.27100 |
Structure solution method | SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.130 | 0.480 |
Number of reflections | 13325 | |
<I/σ(I)> | 5.6 | 1.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 14 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 12% PEG 3350, 0.1M HEPES PH 7.5, 5MM CDCL2 |