2BYG
2nd PDZ Domain of Discs Large Homologue 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-23 |
| Detector | RIGAKU-MSC |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 44.774, 69.081, 73.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.510 - 1.850 |
| R-factor | 0.2 |
| Rwork | 0.196 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pdr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.276 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.540 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.040 | 0.340 |
| Number of reflections | 9967 | |
| <I/σ(I)> | 19.9 | 2.2 |
| Completeness [%] | 98.7 | 91.4 |
| Redundancy | 4.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 25% PEG 3350, 0.2M NACL, 0.1M BIS-TRIS PH 5.5 |
