2BYD
Structure of aminoadipate-semialdehyde dehydrogenase- phosphopantetheinyl transferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-03 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.780, 69.948, 71.239 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.940 - 2.000 |
R-factor | 0.177 |
Rwork | 0.173 |
R-free | 0.24200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.282 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.130 | 0.580 |
Number of reflections | 21391 | |
<I/σ(I)> | 11.8 | 2.27 |
Completeness [%] | 96.4 | 77.6 |
Redundancy | 6.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.7 | 0.05 M H3CIT/NA3CIT PH=5.7 14% PEG3350, pH 5.70 |