2BYC
BlrB - a BLUF protein, dark state structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.600, 65.000, 106.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.28800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.251 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | |
| Number of reflections | 20247 | |
| <I/σ(I)> | 8.6 | 3.9 |
| Completeness [%] | 97.0 | 99 |
| Redundancy | 3.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M ACETATE BUFFER, PH 4.5 0.2 M CALCIUM ACETATE 0.05 M DTT 28 % PEG 400 |
