2BYB
Human Monoamine Oxidase B in complex with Deprenyl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-20 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 132.835, 225.840, 85.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.200 |
R-factor | 0.239 |
Rwork | 0.237 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s2q |
RMSD bond length | 0.017 |
RMSD bond angle | 1.715 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.410 |
Number of reflections | 60540 | |
<I/σ(I)> | 11 | 3 |
Completeness [%] | 95.9 | 98.4 |
Redundancy | 2.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | pH 6.20 |