2BYA
Is radiation damage dependent on the dose-rate used during macromolecular crystallography data collection
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.274, 58.359, 66.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.300 |
| R-factor | 0.109 |
| Rwork | 0.108 |
| R-free | 0.14200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.459 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.230 |
| Number of reflections | 52141 | |
| <I/σ(I)> | 0 | 3.6 |
| Completeness [%] | 98.8 | 93 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 8000, 0.2 M AMMONIUM SULPHATE AND 0.1 M TRIS-HCL PH 8.0 |
