2BY3
Is radiation damage dependent on the dose-rate used during macromolecular crystallography data collection
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.505, 66.645, 152.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.20400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.831 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.110 | 1.090 |
| Number of reflections | 98176 | |
| <I/σ(I)> | 9.5 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 2000 MME, 0.1 M TRIS-HCL PH 8.5, 5% GLYCEROL AND 0.1 M MGCL2 |
