2BXR
Human Monoamine Oxidase A in complex with Clorgyline, Crystal Form A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Collection date | 2005-02-14 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.493, 109.599, 81.329 |
| Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
| Resolution | 87.040 - 3.000 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2q |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.696 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.010 | 0.520 |
| Number of reflections | 23085 | |
| Completeness [%] | 96.9 | 97.3 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 6% PEG 6000, 100 MM LISULPHATE, 100 MM NACITRATE, 50 MM KPI PH 7.0. |
