2BX9
Crystal structure of B.subtilis Anti-TRAP protein, an antagonist of TRAP-RNA interactions
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.87, 1.28258 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.552, 60.125, 60.348 |
| Unit cell angles | 113.99, 101.37, 100.50 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.197 |
| Rwork | 0.192 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0013) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.180 |
| Number of reflections | 14370 | |
| <I/σ(I)> | 5.7 | 2.4 |
| Completeness [%] | 93.4 | 83 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | CONDITIONS: 21-23 % (W/V) POLY(ETHYLENE) GLYCOL 5000 MONMETHYL ETHER; 0.1 M SODIUM CACODYLATE PH 6.5; 50 MM MAGNESIUM ACETATE CRYOSOLUTION: 16 % GLYCEROL (V/V) WITH HIGHER CONCENTRATION OF POLY(ETHYLENE) GLYCOL |
