2BX9
Crystal structure of B.subtilis Anti-TRAP protein, an antagonist of TRAP-RNA interactions
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2003-06-27 |
Detector | ADSC CCD |
Wavelength(s) | 0.87, 1.28258 |
Spacegroup name | P 1 |
Unit cell lengths | 51.552, 60.125, 60.348 |
Unit cell angles | 113.99, 101.37, 100.50 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.197 |
Rwork | 0.192 |
R-free | 0.29600 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.369 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0013) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.110 | 0.180 |
Number of reflections | 14370 | |
<I/σ(I)> | 5.7 | 2.4 |
Completeness [%] | 93.4 | 83 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | CONDITIONS: 21-23 % (W/V) POLY(ETHYLENE) GLYCOL 5000 MONMETHYL ETHER; 0.1 M SODIUM CACODYLATE PH 6.5; 50 MM MAGNESIUM ACETATE CRYOSOLUTION: 16 % GLYCEROL (V/V) WITH HIGHER CONCENTRATION OF POLY(ETHYLENE) GLYCOL |