2BX2
Catalytic domain of E. coli RNase E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 195.838, 195.838, 143.569 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.850 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.551 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.850 |
| Rmerge | 0.100 | 0.810 |
| Number of reflections | 36133 | |
| <I/σ(I)> | 17.9 | 3.5 |
| Completeness [%] | 97.4 | 96.7 |
| Redundancy | 9.2 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | CRYSTALS OF THE RNASE E CATALYTIC DOMAIN/ RNA COMPLEX APPEARED AFTER TWO TO FOUR WEEKS IN 5 TO 20 % WT/V POLYETHYLENE GLYCOL 8,000, 0.1 M TRIS PH 7.5 TO 8.0, AND 10 TO 50 MM MAGNESIUM FORMATE AT 20OC |
